Q. No.Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
1. Bioreactor 2. BOD incubator 3. Sludge digester 4. Industrial oven
DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol
3. Chilled ethanol
4. Methanol at room temperature
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
| 1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
| 2. | The enzyme cuts DNA molecules at an identified position within the DNA |
| 3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
| 4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
| 1. | cutting of DNA into fragments |
| 2. | separation of DNA fragments according to their size |
| 3. | construction of recombinant DNA by joining with cloning vectors |
| 4. | isolation of DNA molecule |
Stirred-tank bioreactors have been designed for:
1. Addition of preservatives to the product
2. Purification of the product
3. Ensuring anaerobic conditions in the culture vessel
4. Availability of oxygen throughout the process
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
1. Coelom
2. Coenzyme
3. Coli
4. Colon
5’___GAATTC___3’
3’___CTTAAG__5’
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:
1. Silver or platinum
2. Platinum or zinc
3. Silicon or platinum
4. Gold or tungsten
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