Genetic engineering involves:
1. Use of restriction endonuclease on bacterial DNA and formation of new traits
2. Use of Ligase for cutting DNA
3. Developing instruments
4. Use of statistic in genetics

Restriction endonucleases:

Which of the following enzymes are used to join bits of DNA:

1. Ligase

2. Primase

3. DNA polymerase

4. Endonuclease

Manipulation of DNA in genetic engineering became possible due to the discovery of:

1. Restriction endonuclease

2. DNA ligase

3. Transcriptase

4. Primase

In Lederberg's replica plating experiment, what shall be used to obtain streptomycin resistant strain?
1.  Minimal medium and streptomycin
2.  Complete medium and streptomycin
3.  Only minimal medium
4.  Only complete medium

DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

1. Extension, Denaturation, Annealing

2. Annealing, Denaturation, Extension

3. Denaturation, Annealing, Extension

4. Denaturation, Extension, Annealing

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

Plasmid pBR322 has a PstI restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\mathrm{\beta }$-galactoside production and the recombinant plasmid is inserted in an E.coli strain,

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is: 

1. Palindromic Nucleotide sequences

2. Poly(A) tail sequences

3. Degenerate primer sequence

4. Okazaki sequences