Q. No.In genetic engineering, the antibiotic-resistance gene is used:
1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers
1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers
Which of the following are used in gene cloning:
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleoids
In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:
| A. | a bacterium | B. | plasmid |
| C. | plasmodium | D. | bacteriophage |
Options:
| 1. | (A) only | 2. | (A) and (C) only |
| 3. | (B) and (D) only | 4. | (A), (B) and (D) only |
Which one of the following techniques made it possible to genetically engineer living organisms?
1. Hybridization
2. Recombinant DNA techniques
3. X-ray diffraction
4. Heavier isotope labelling
Genetic engineering involves:
1. Use of restriction endonuclease on bacterial DNA and formation of new traits
2. Use of Ligase for cutting DNA
3. Developing instruments
4. Use of statistic in genetics
Restriction endonucleases:
| 1. | Are used in genetic engineering for ligating two DNA molecules. |
| 2. | Are used for in vitro DNA synthesis. |
| 3. | Are synthesized by bacteria as part of their defense mechanism. |
| 4. | Are present in mammalian cells for the degradation of DNA when the cell dies. |
Which of the following enzymes are used to join bits of DNA:
1. Ligase
2. Primase
3. DNA polymerase
4. Endonuclease
Manipulation of DNA in genetic engineering became possible due to the discovery of:
1. Restriction endonuclease
2. DNA ligase
3. Transcriptase
4. Primase
Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
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