Q. No.Match the following enzymes with their functions:
Column-I
Column-II
(a)
Restriction
endonuclease
(i)
joins the DNA fragments
(b)
Exonuclease
(ii)
extends primers on genomic DNA template
(c)
DNA ligase
(iii)
cuts DNA at a specific position
(d)
Tag polymerase
(iv)
removes nucleotides from the ends of DNA
Select the correct option from the following:
Options:
(a)
(b)
(c)
(d)
1.
(iii)
(i)
(iv)
(ii)
2.
(iii)
(iv)
(i)
(ii)
3.
(iv)
(iii)
(i)
(ii)
4.
(ii)
(iv)
(i)
(iii)
Column-I
Column-II
(a)
Restriction
endonuclease
(i)
joins the DNA fragments
(b)
Exonuclease
(ii)
extends primers on genomic DNA template
(c)
DNA ligase
(iii)
cuts DNA at a specific position
(d)
Tag polymerase
(iv)
removes nucleotides from the ends of DNA
Select the correct option from the following:
The two antibiotic resistance genes on vector pBR 322 are for:
1. Ampicillin and Tetracycline
2. Ampicillin and Chloramphenicol
3. Chloramphenicol and Tetracycline
4. Tetracycline and Kanamycin
A selectable marker is used to:
| 1. | help in eliminating the non-transformants so that the transformants can be regenerated. |
| 2. | identify the gene for the desired trait in an alien organism. |
| 3. | select a suitable vector for transformation in a specific crop. |
| 4. | mark a gene on a chromosome for isolation using a restriction enzyme. |
Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:
| (a) | DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal. |
| (b) | DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA. |
| (c) | The smaller the DNA fragment, the greater the distance it travels through it. |
| (d) | Pure DNA can be visualized directly by exposing it to UV radiation. |
Choose the correct answer from the options given below:
| 1. | (a), (c) and (d) | 2. | (a), (b) and (c) |
| 3. | (b), (c) and (d) | 4. | (a), (b) and (d) |
An enzyme catalysing the removal of nucleotides from ends of DNA is:
1. DNA ligase
2. Endonuclease
3. Exonuclease
4. Protease
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
| 1. | Bioreactor | 2. | BOD incubator |
| 3. | Sludge digester | 4. | Industrial oven |
DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol
3. Chilled ethanol
4. Methanol at room temperature
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
| 1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
| 2. | The enzyme cuts DNA molecules at an identified position within the DNA |
| 3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
| 4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
| 1. | cutting of DNA into fragments |
| 2. | separation of DNA fragments according to their size |
| 3. | construction of recombinant DNA by joining with cloning vectors |
| 4. | isolation of DNA molecule |
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