Given below are the three basic steps in genetically modifying an organism

a. Introduction of the identified DNA into the host.

b. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny.

c. Identification of DNA with desirable genes.

Choose the option with respect to correct sequence of the given steps

(1) a c b    

(2) c b a

(3) c a b    

(4) b a c

Subtopic:  General Design of an rDNA experiment |
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Following enzymes/techniques are used in the process of recombinant DNA technology

a. EcoRl to cut the isolated genome

b. DNA ligase

c. Protease and ribonuclease for removal of proteins and RNA from DNA

d. Production of recombinant hosts

e. Lysozyme for isolation of the genetic material (DNA)

f. Gel electrophoresis for separation and isolation of DNA fragments

Mark the correct sequence of their use

(1) c, e, b, f, a, d               

(2) e, c, a, b, f, d

(3) e, c, a, f, b, d

(4) a, e, c, b, d, f

Subtopic:  General Design of an rDNA experiment |
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What is true regarding A, B and C in the given diagrammatic representation of rDNA technology?
  

I:  At A, same restriction enzyme is used to cut both foreign and vector DNA
II:  The enzyme used at B is DNA ligase
III:  Step C can be called as transformation
 
1. I and II only 2. I and III only
3. II and III only 4. I, II and III
Subtopic:  General Design of an rDNA experiment |
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