Two bacteria found to be very useful in genetic engineering are

Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
a. Zinc or tungsten
b. Silicon or gold
c. Tungsten or gold
d. Selenium or Platinum

Given below is a sample of portion of DNA strand giving the base sequence on the

opposite strands. What is so, special shown in it?

5'__ GAATTC_ 3'

3' __ CTTAAG__5'

1. Deletion mutation

2. Start codon at the 5' end

3. Palindromic sequence of base pairs

4. Replication completed

For transformation, microparticles coated with DNA to be bombarded with gene gun are

made up of

1. silver or platinum

2. platinum or zinc

3. silicon or platinum

4. gold or tungsten

____________ is a thermophilic bacterium that can survive temperatures upto $95°$C. Select the option which fills the blank correctly.

1. Thermus aquaticus

2. Salmonella typhi

3. E.coli

4. Lactobacillus

Which of these are most widely used in genetic engineering?

1. Plastid

2. Plasmid

3. Mitochondrion

4. Ribosome

The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

Which one of the following palindromic base sequences in DNA can be easily cut at about

the middle by some particular restriction enzyme?

1. 5'$-$CGTTCG$-$3'

     3'$-$ATGGTA$-$-5'

2. 5'$-$GATATG$-$3'

     3'$-$CTACTA$-$5'

3. 5'$-$GAATTC$-$3'

     3'$-$CTTAAG$-$5'

4. 5'$-$CACGTA$-$3'

     3'$-$CTCAGT$-$5'

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

1. Trichoderma harzianum

2. Meloidogyne incognita

3. Agrobacterium tumefaciens

4. Penicillium expansum

Which one of the following represents a palindromic sequence in DNA?  
1. 5' - GAATTC - 3'
    3' - CTTAAG - 5'

2. 5' - CCAATG - 3'
    3' - GAATCC - 5'

3. 5' - CATTAG - 3'
    3' - GATAAC - 5'

4. 5' - GATACC - 3'
    3' - CCTAAG - 5'