'Insertional inactivation' is used for

(1) Selection of recombinants

(2) Producing pest resistant crops

(3) Enhancing nutritional value of food

(4) Reducing post harvest losses

Subtopic:  Selection of Recombinant Transformants |
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Selection of recombinants is based on all except one. Find out the exception

(1) Expression and non-expression of genes encoding for tetracycline-resistant compound

(2) Expression and non-expression of genes encoding for insulin-resistant compound

(3) Expression and non-expression of genes encoding for ampicillin-resistant compound

(4) Insertional inactivation

Subtopic:  Selection of Recombinant Transformants |
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When recombinant DNA is inserted within the coding sequence of an enzyme β-galactosidase. This results in the following except

1. Insertional inactivation

2. Recombinant colonies do not produce any color

3. Inactivation of enzyme

4. Chromogenic substrate is converted into product by recombinant colonies

Subtopic:  Selection of Recombinant Transformants |
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If a foreign gene is inserted into pBR322 by using restriction enzyme BamHI, the selection process, for identifying recombinants requires plating on all except

1. Ampicillin rich medium

2. Tetracycline rich medium

3. X-gal rich medium

4. Nutrient medium without antibiotics

Subtopic:  Selection of Recombinant Transformants |
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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

(1) Non-recombinant bacteria containing β-galactosidase

(2) Insertional inactivation of α-galactosidase in non-recombinant bacteria

(3) Insertional inactivation of β-galactosidase in recombinant bacteria

(4) Inactivation of glycosidase enzyme in recombinant bacteria

 

Subtopic:  Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
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