- 1(current)
Q. No.Which of the following are used in gene cloning:
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleoids
The vector used to transfer gene to produce pest resistant tobacco plant is
(1) pBR 322
(2) pUC 18
(3) Ti plasmid
(4) λ phage
Part of a plasmid such as pBR322 responsible for controlling the copy number of the linked alien DNA is
1. Ori
2. rop
3. ampR
4. tetR
rop segment codes for pBR322?
1. Resistance protein
2. Protein for replication
3. Proteins for translation
4. Cloning site
In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:
| A. | a bacterium | B. | plasmid |
| C. | plasmodium | D. | bacteriophage |
Options:
| 1. | (A) only | 2. | (A) and (C) only |
| 3. | (B) and (D) only | 4. | (A), (B) and (D) only |
A and B in pBR322, shown in the diagram given below, respectively represent recognition sequences of:
| 1. | BamH I and Sma I | 2. | Hind II and Sma I |
| 3. | BamH I and Sal I | 4. | Sal I and Hind I |
Which of the following options is correct about Agrobacterium tumifaciens?
| 1. | It is a pathogen of angiosperms |
| 2. | It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen |
| 3. | Its Ti plasmid is now modified into an expression vector |
| 4. | Its Ti plasmid is modified into a cloning vector which is no more pathogenic |
The two antibiotic resistance genes on vector pBR 322 are for:
1. Ampicillin and Tetracycline
2. Ampicillin and Chloramphenicol
3. Chloramphenicol and Tetracycline
4. Tetracycline and Kanamycin
- 1(current)
Q. No.
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