Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:

(a) DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal.
(b) DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA.
(c) The smaller the DNA fragment, the greater the distance it travels through it.
(d) Pure DNA can be visualized directly by exposing it to UV radiation.


Choose the correct answer from the options given below:

1. (a), (c) and (d) 2. (a), (b) and (c)
3. (b), (c) and (d) 4. (a), (b) and (d)
Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
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Gel electrophoresis is used for

1. cutting of DNA into fragments

2. separation of DNA fragments according to their size

3. construction of recombinant DNA by joining with cloning vectors

4. isolation of DNA molecule

Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
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In rDNA technology or genetic engineering elution means :-

1. Remove the DNA from centrifuge tube after centrifugation

2. Separation of the recombinant protein from recombinant cell

3. Insertion of recombinant DNA into host cell

4. The separated band of DNA are cut out from the gel and extracted from the gel piece

Subtopic:  Separation and Isolation of DNA fragments |
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Which of the following carries a natural plasmid that can most effectively and exclusively be used in transforming plant cell only?

(1) Meloidogyne incognita

(2) Bacillus thuringiensis

(3) Agrobacterium tumifaciens

(4) Escherichia coil

Subtopic:  Separation and Isolation of DNA fragments |
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In the above diagram showing a typical agaro gel
electrophoresis

1. Lane 1 shows undigested DNA fragment

2. Lane 4 shows largest DNA particles towards ‘y’

3. The procedure helps to form clones of DNA

4. The procedure takes place as the DNA fragments
are double stranded

Subtopic:  Separation and Isolation of DNA fragments |
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DNA fragments separated by gel electrophoresis are shown. Mark the correct statement :

 

1. Band 3 contains more positively charged DNA molecules than band 1.
2. Band 3 indicates more charge density than bands 1 and 2.
3. Band 1 has longer DNA fragment than bands 2 and 3.
4. All bands have equal lengths and charges but differ in base composition.
Subtopic:  Separation and Isolation of DNA fragments |
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During the process of gel electrophoresis, DNA moves towards the _A_ of the electrophoretic chamber as the charge of DNA is _B_.
Choose the option that fills the blanks correctly.

         A               B
1. Cathode       Positive
2. Cathode      Negative
3. Anode        Positive
4. Anode        Negative

Subtopic:  Separation and Isolation of DNA fragments |
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