Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?

1.	Bioreactor	2.	BOD incubator
3.	Sludge digester	4.	Industrial oven

The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. EcoRI

2. HindII

3. SmaI

4. BamHI

The following palindrome is recognized by the restriction enzyme:

1. BamHI

2. EcoRI

3. HindII

4. PstI

Which of the following does not have the ability to replicate within bacterial cells independent of the control of chromosomal DNA?
1. Plastid

2. Bacteriophages

3. BAC

4. Plasmid

The first recombinant DNA was constructed
by linking an antibiotic resistant gene with the
native plasmid of
1. Escherichia coli
2. Salmonella typhimurium
3. Clostridium butylicum
4. Acetobacter aceti

____________ is a thermophilic bacterium that can survive temperatures upto $95°$C. Select the option which fills the blank correctly.

1. Thermus aquaticus

2. Salmonella typhi

3. E.coli

4. Lactobacillus

Select the two core techniques that enabled the birth of modern biotechnology:

1. Genetic engineering and bioprocess engineering

2. Genetic engineering and biolistics

3. Chemical engineering and biopiracy

4. Downstream processing and bioprocess engineering

Select the correct statement .

1. A restriction exonuclease cuts both strands of foreign DNA as well as vector DNA at specific palindromic sequences

2. A restriction endonuclease is named on the basis of scientific name and strain of bacteria from which it is isolated

3. All restriction enzymes cut the strands of DNA in the centre of palindromic site between the same two bases of opposite strand

4. When cut by same restriction enzyme, the resultant DNA fragments generate different kinds of sticky ends

Separation and purification by filtration, centrifugation of desired compound produced in bioreactor is a part of

1. Downstream processing only

2. Scaling up and downstream processing

3. Upstream processing

4. Screening for recombinants and downstream processing

Which of the following is not a method of introducing alien DNA into host cells?

1. Microinjection

2. Heat shock method

3. Being placed along with the cell into a gene gun

4. Gel electrophoresis