Q. No.A and B in pBR322, shown in the diagram given below, respectively represent recognition sequences of:
1. BamH I and Sma I
2. Hind II and Sma I
3. BamH I and Sal I
4. Sal I and Hind I
What is true regarding A, B and C in the given diagrammatic representation of rDNA technology?
| I: | At A, same restriction enzyme is used to cut both foreign and vector DNA |
| II: | The enzyme used at B is DNA ligase |
| III: | Step C can be called as transformation |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II and III |
Consider the following statements:
| I: | Biolistics or gene gun can be used to introduce genes in both plant and animal cells. |
| II: | Agrobacterium tumefaciens does not naturally infect Monocots. |
| III: | Liposomes are used in gene therapy for cystic fibrosis. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Consider the following statements:
| I: | Ori is responsible for the copy number of the linked DNA. |
| II: | Transformation is a process through which a piece of DNA is introduced into a host bacterium. |
| III: | Bacteriophages have very high copy numbers of their genome within the bacterial cell. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Consider the following statements:
| I: | Exonucleases remove nucleotides from the ends of DNA whereas, endonucleases make cuts at specific positions within DNA. |
| II: | To visualize pure DNA fragments, it is stained with ethidium bromide and seen in UV light. |
| III: | Plasmids and phage DNA are used as vectors in genetic engineering as they are extrachromosomal DNA molecules. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Consider the following statements:
| I: | Asexual reproduction preserves genetic information while sexual reproduction permits variations. |
| II: | Traditional hybridization often leads to the inclusion and multiplication of undesirable genes along with the desired genes. |
| III: | rDNA technology allows us to isolate and introduce only one or a set of desirable genes without introducing undesirable genes in the target organism. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
For the multiplication of any alien piece of DNA in an organism:
1. It must be devoid of any introns in it
2. It must be a part of a chromosome with an ori
3. It must have recognition sequence of at least one restriction enzyme
4. It must have selectable markers
For the synthesis of 4 copies of DNA, how many sets of primers are needed in PCR
1. 2 sets
2. 8 sets
3. 4 sets
4. Random
The process of competent cell formation include
1. CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation
2. Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA
3. CaCl2 treatment - Heat shock -Ice incubation with r-DNA
4. Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA
In order to make the host cell competent
Divalent cations such as calcium are used-
1. The divalent ions have to be in a specific concentration
2. They cause the DNA uptake by the cell
3. They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.
4. All of the above
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