If any protein-encoding gene is expressed in a heterologous host, it is termed as

1. Recombinant protein

2. Single-cell protein

3. Humulin

4. Transposon

Subtopic:  Process of Biotech |
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Read the following statements

Statement-A : Restriction enzymes are obtained only from eukaryotes.

Statement-B : The first discovered restriction endonuclease was EcoRI.

Choose the correct option.

(1) Both statements are correct

(2) Both statements are incorrect

(3) Statement A is correct but B is incorrect

(4) Statement B is correct but A is incorrect

Subtopic:  Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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Genetically engineered plants are generated in laboratory by altering their genetic makeup. This is done by adding one or more genes to a plant’s genome by

(1) Biolistic method or Agrobacterium tumefaciens mediated transformation

(2) DNA injection or heat shock method

(3) Fusing liposomes to intact plant cells

(4) Bacillus thuringiensis mediated transformation

Subtopic:  Transforming Plant & Animal Cell |
 85%
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The enzyme known as molecular scissors in genetic engineering is

1. DNA ligase

2. Transferases

3. DNA polymerase

4. Restriction endonuclease

Subtopic:  Restriction Enzymes - Main Enzymes |
 95%
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During the process of gel electrophoresis, DNA moves towards the _A_ of the electrophoretic chamber as the charge of DNA is _B_.
Choose the option that fills the blanks correctly.

         A               B
1. Cathode       Positive
2. Cathode      Negative
3. Anode        Positive
4. Anode        Negative

Subtopic:  Separation and Isolation of DNA fragments |
 75%
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If a foreign gene is inserted into pBR322 by using restriction enzyme BamHI, the selection process, for identifying recombinants requires plating on all except

1. Ampicillin rich medium

2. Tetracycline rich medium

3. X-gal rich medium

4. Nutrient medium without antibiotics

Subtopic:  Selection of Recombinant Transformants |
From NCERT
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Downstream processing does not involve

1. Biosynthesis

2. Separation

3. Purification

4. Centrifugation

Subtopic:  Bioreactors/Downstream Processing |
 59%
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All the following are transformation procedures except

1. Microinjection

2. Biolistics

3. Spooling

4. Electroporation

Subtopic:  Transforming Plant & Animal Cell |
 90%
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Select the incorrect statement.

1. Separation of DNA fragments occurs based on their size in agarose gel.

2. Blue-white selection involves insertional inactivation of β-galactosidase.

3. Treatment with ice-cold calcium can enhance efficiency of transformation in host cells.

4. Extension step during PCR is based on thermolabile nature of Taq polymerase.

Subtopic:  Polymerase Chain Reaction: PCR |
 52%
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DNA from Agrobacterium tumefaciens can be extracted through use of enzyme

1. Chitinase

2. Cellulase

3. Lysozyme

4. DNAse

Subtopic:  Host & Desired DNA |
 61%
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