Modern biotechnology consist:-

1. Microbiology

2. Tissue culture

3. Genetic engineering

4. All the above

In rDNA technology or genetic engineering elution means :-

1. Remove the DNA from centrifuge tube after centrifugation

2. Separation of the recombinant protein from recombinant cell

3. Insertion of recombinant DNA into host cell

4. The separated band of DNA are cut out from the gel and extracted from the gel piece

If a DNA fragment is cut by EcoRI at a specific recognition site then

1. Blunt-end cannot be obtained

2. Sticky-ends cannot be obtained

3. Construction of rDNA would not be possible

4. The plasmid with one site get cut into two fragments

The enzyme that seals 5’ PO4 and 3’ OH polynucleotide ends while creating a recombinant DNA molecule is

1. Alkaline phosphatase

2. DNA ligase

3. DNAse

4. Restriction endonuclease

Part of a plasmid such as pBR322 responsible for controlling the copy number of the linked alien DNA is

1. Ori

2. rop

3. amp^R

4. tet^R

DNA from Agrobacterium tumefaciens can be extracted through use of enzyme

1. Chitinase

2. Cellulase

3. Lysozyme

4. DNAse

Select the incorrect statement.

1. Separation of DNA fragments occurs based on their size in agarose gel.

2. Blue-white selection involves insertional inactivation of β-galactosidase.

3. Treatment with ice-cold calcium can enhance efficiency of transformation in host cells.

4. Extension step during PCR is based on thermolabile nature of Taq polymerase.

All the following are transformation procedures except

1. Microinjection

2. Biolistics

3. Spooling

4. Electroporation

Downstream processing does not involve

1. Biosynthesis

2. Separation

3. Purification

4. Centrifugation

To transform a bacterial cell with recombinant DNA, which of the following is required?

(1) 90°C temperature

(2) Divalent calcium ions

(3) Selectable marker

(4) Restriction enzyme digested DNA