Q. No.Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:
(a)
DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal.
(b)
DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA.
(c)
The smaller the DNA fragment, the greater the distance it travels through it.
(d)
Pure DNA can be visualized directly by exposing it to UV radiation.
Choose the correct answer from the options given below:
1.
(a), (c) and (d)
2.
(a), (b) and (c)
3.
(b), (c) and (d)
4.
(a), (b) and (d)
Choose the correct answer from the options given below:
Which one of the following represents a palindromic sequence in DNA?
1. 5' - GAATTC - 3'
3' - CTTAAG - 5'
2. 5' - CCAATG - 3'
3' - GAATCC - 5'
3. 5' - CATTAG - 3'
3' - GATAAC - 5'
4. 5' - GATACC - 3'
3' - CCTAAG - 5'
In the above diagram showing a typical agaro gel
electrophoresis
1. Lane 1 shows undigested DNA fragment
2. Lane 4 shows largest DNA particles towards ‘y’
3. The procedure helps to form clones of DNA
4. The procedure takes place as the DNA fragments
are double stranded
A selectable marker is used to:
| 1. | help in eliminating the non-transformants so that the transformants can be regenerated. |
| 2. | identify the gene for the desired trait in an alien organism. |
| 3. | select a suitable vector for transformation in a specific crop. |
| 4. | mark a gene on a chromosome for isolation using a restriction enzyme. |
The two antibiotic resistance genes on vector pBR 322 are for:
1. Ampicillin and Tetracycline
2. Ampicillin and Chloramphenicol
3. Chloramphenicol and Tetracycline
4. Tetracycline and Kanamycin
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | (iii) | (i) | (iv) | (ii) |
| 2. | (iii) | (iv) | (i) | (ii) |
| 3. | (iv) | (iii) | (i) | (ii) |
| 4. | (ii) | (iv) | (i) | (iii) |
The product has to be subjected through a series of processes before it is ready for marketing as a finished product called down stream processing. It includes
1. Separation
2. Purification
3. Quality control
4. Both 1 & 2
Similarity between microinjection and biolistic gene gun method is
| 1. | Used to transform the plant host cells. |
| 2. | Used to transform the animal host cells. |
| 3. | Recombinant DNA is directly injected into host cells. |
| 4. | Used to transform the bacterial host cells |
Which of the following enzyme is involved in the isolation of genetic material like DNA from a plant cell?
| I: | Cellulase |
| II: | Lysozyme |
| III: | Ribonuclease |
| IV: | Protease |
1. I, II, III and IV
2. I and II only
3. I only
4. I, III and IV
If the gene of interest is cloned at ECoRI in pBR322,
the recombinant E-coli after transformation are
1. Susceptible to ampicillin and tetracyline.
2. Sensitive to tetracycline.
3. Resistant to kanamycin.
4. Resistant to ampicillin and tetracycline
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