- 1(current)
Q. No.Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
1. D and E only
2. B and C only
3. C and D only
4. A and B only
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
1. D and E only
2. B and C only
3. C and D only
4. A and B only
Subtopic: Â Tools |
 75%
Level 2: 60%+
NEET - 2025
Please attempt this question first.
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Please attempt this question first.
Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) only2. (c), (d) and (e) only
3. (a), (b) and (c) only
4. (a), (c), (d) and (e) only
Subtopic: Â Cloning Vector |Â Tools |
 87%
Level 1: 80%+
NEET - 2022
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The sequence that controls the copy number of the linked DNA in the vector is termed as :
1. Ori site
2. Palindromic sequence
3. Recognition site
4. Selectable marker
2. Palindromic sequence
3. Recognition site
4. Selectable marker
Subtopic: Â Tools: Vectors: I |Â Tools |
 80%
Level 1: 80%+
NEET - 2020
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The specific palindromic sequence which is recognized by EcoRI is:
| 1. | 5' -GGAACC-3' 3'-CCTTGG-5' |
2. | 5'-CTTAAG-3' 3'-GAATTC-5' |
| 3. | 5'-GGATCC-3' 3'-CCTAGG-5' |
4. | 5'-GAATTC-3' 3'-CTTAAG-5' |
Subtopic: Â Tools: Enzymes: I |Â Restriction Enzymes - Main Enzymes |Â Tools |
 83%
Level 1: 80%+
NEET - 2020
Hints
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | (iii) | (i) | (iv) | (ii) |
| 2. | (iii) | (iv) | (i) | (ii) |
| 3. | (iv) | (iii) | (i) | (ii) |
| 4. | (ii) | (iv) | (i) | (iii) |
Subtopic: Â Tools: Enzymes: I |Â Tools: Enzymes: II |Â Tools |
 91%
Level 1: 80%+
NEET - 2019
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Links
An enzyme catalysing the removal of nucleotides from ends of DNA is:
| 1. | DNA ligase | 2. | Endonuclease |
| 3. | Exonuclease | 4. | Protease |
Subtopic: Â Tools: Enzymes: I |Â Tools |
 88%
Level 1: 80%+
NEET - 2019
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- 1(current)
Q. No.
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