Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
1. D and E only
2. B and C only
3. C and D only
4. A and B only 
Subtopic:  Tools |
 77%
From NCERT
NEET - 2025
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Identify the properties of a good vector used in rDNA technology:

(a) It should have origin of replication supporting a high copy number 
(b) It should have preferably more than '2' recognition sites
(c) The restriction sites in vector should be in the antibiotic- resistant genes 
(d) It should have suitable marker genes 
(e) It should be easy to isolate and purify 

Choose the most appropriate answer from the options given below: 

1. (a), (c) and (e) only 
2. (c), (d) and (e) only 
3. (a), (b) and (c) only 
4. (a), (c), (d) and (e) only 
Subtopic:  Cloning Vector | Tools |
 87%
From NCERT
NEET - 2022
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The sequence that controls the copy number of the linked DNA in the vector is termed as :
1. Ori site
2. Palindromic sequence
3. Recognition site
4. Selectable marker
Subtopic:  Tools: Vectors: I | Tools |
 80%
From NCERT
NEET - 2020
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The specific palindromic sequence which is recognized by EcoRI is:

1. 5' -GGAACC-3'
3'-CCTTGG-5'
2. 5'-CTTAAG-3'
3'-GAATTC-5'
3. 5'-GGATCC-3'
3'-CCTAGG-5'
4. 5'-GAATTC-3'
3'-CTTAAG-5'
Subtopic:  Tools: Enzymes: I | Restriction Enzymes - Main Enzymes | Tools |
 83%
From NCERT
NEET - 2020
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Match the following enzymes with their functions:
 

Column-I

Column-II

(a)

Restriction

endonuclease

(i)

joins the DNA fragments

(b)

Exonuclease

(ii)

extends primers on genomic DNA template

(c)

DNA ligase

(iii)

cuts DNA at a specific position

(d)

Tag polymerase

(iv)

removes nucleotides from the ends of DNA


Select the correct option from the following:

Options: (a) (b) (c) (d)
1. (iii) (i) (iv) (ii)
2. (iii) (iv) (i) (ii)
3. (iv) (iii) (i) (ii)
4. (ii) (iv) (i) (iii)

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II | Tools |
 91%
From NCERT
NEET - 2019
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An enzyme catalysing the removal of nucleotides from ends of DNA is:

1. DNA ligase 2. Endonuclease
3. Exonuclease 4. Protease
Subtopic:  Tools: Enzymes: I | Tools |
 88%
From NCERT
NEET - 2019
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