Identify the properties of a good vector used in rDNA technology:

(a) It should have origin of replication supporting a high copy number 
(b) It should have preferably more than '2' recognition sites
(c) The restriction sites in vector should be in the antibiotic- resistant genes 
(d) It should have suitable marker genes 
(e) It should be easy to isolate and purify 


Choose the most appropriate answer from the options given below: 

1. (a), (c) and (e) only  2. (c), (d) and (e) only 
3. (a), (b) and (c) only  4. (a), (c), (d) and (e) only 
Subtopic:  Cloning Vector | Tools |
 86%
From NCERT
NEET - 2022
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh
Hints
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh

The sequence that controls the copy number of the linked DNA in the vector is termed as :
1. Ori site
2. Palindromic sequence
3. Recognition site
4. Selectable marker
Subtopic:  Tools: Vectors: I | Tools |
 77%
From NCERT
NEET - 2020
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh
Hints
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh

The specific palindromic sequence which is recognized by EcoRI is:

1. 5' -GGAACC-3'
3'-CCTTGG-5'
2. 5'-CTTAAG-3'
3'-GAATTC-5'
3. 5'-GGATCC-3'
3'-CCTAGG-5'
4. 5'-GAATTC-3'
3'-CTTAAG-5'
Subtopic:  Tools: Enzymes: I | Restriction Enzymes - Main Enzymes | Tools |
 82%
From NCERT
NEET - 2020
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh
Hints
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh

advertisementadvertisement

Match the following enzymes with their functions:
 

Column-I

Column-II

(a)

Restriction

endonuclease

(i)

joins the DNA fragments

(b)

Exonuclease

(ii)

extends primers on genomic DNA template

(c)

DNA ligase

(iii)

cuts DNA at a specific position

(d)

Tag polymerase

(iv)

removes nucleotides from the ends of DNA


Select the correct option from the following:

Options: (a) (b) (c) (d)
1. (iii) (i) (iv) (ii)
2. (iii) (iv) (i) (ii)
3. (iv) (iii) (i) (ii)
4. (ii) (iv) (i) (iii)

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II | Tools |
 90%
From NCERT
NEET - 2019
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh

An enzyme catalysing the removal of nucleotides from ends of DNA is:
1. DNA ligase
2. Endonuclease
3. Exonuclease
4. Protease

Subtopic:  Tools: Enzymes: I | Tools |
 88%
From NCERT
NEET - 2019
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh
Hints
Links
To view explanation, please take trial in the course.
NEET 2023 - Target Batch - Aryan Raj Singh