In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
1. White color colonies will be selected.
2. Blue color colonies grown on ampicillin plates can be selected.
3. Using ampicillin & tetracyclin containing medium plate.
4. Blue color colonies will be selected.
Subtopic:  Cloning Vector |
 64%
From NCERT
NEET - 2025
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
1. The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.
2. The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics.
3. Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance.
4. The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid.
Subtopic:  Cloning Vector |
 75%
From NCERT
NEET - 2024
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic:  Cloning Vector |
 89%
From NCERT
NEET - 2024
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Match List-I with List-II
List-I List-II
A.  Ampicillin  I. Restriction sites 
B.  Ori II. Blue/White screening
C.  Multiple cloning sites III. Selectable marker 
D.  \(\beta\) galactosidase gene IV. Sequence from where replication starts
Choose the correct answer from the options given below: 
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
Subtopic:  Cloning Vector |
 85%
From NCERT
NEET - 2024
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic:  Cloning Vector |
 85%
From NCERT
NEET - 2024
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
1. PsT I 2. Pvu I
3. EcoR I 4. BamH I
Subtopic:  Cloning Vector |
 85%
From NCERT
NEET - 2023
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 Match List-I with List-II
List-I List-II
(A) Kanamycin (I) Delivers genes into animal cells
(B) ClaI (II) Selectable marker
(C) Disarmed retroviruses (III) Restriction site
(D) Kanamycin Rgene (IV) Antibiotic resistance

Choose the correct answer from the options given below:
Options: (A) (B) (C) (D)
1. (II) (III) (I) (IV)
2. (III) (I) (IV) (II)
3. (IV) (III) (I) (II)
4. (II) (IV) (I) (III)
Subtopic:  Cloning Vector |
 55%
From NCERT
NEET - 2023
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Which  of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Subtopic:  Cloning Vector |
 75%
From NCERT
NEET - 2022
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Identify the properties of a good vector used in rDNA technology:

(a) It should have origin of replication supporting a high copy number 
(b) It should have preferably more than '2' recognition sites
(c) The restriction sites in vector should be in the antibiotic- resistant genes 
(d) It should have suitable marker genes 
(e) It should be easy to isolate and purify 

Choose the most appropriate answer from the options given below: 

1. (a), (c) and (e) only 
2. (c), (d) and (e) only 
3. (a), (b) and (c) only 
4. (a), (c), (d) and (e) only 
Subtopic:  Cloning Vector | Tools |
 87%
From NCERT
NEET - 2022
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Pathogenic bacteria gain resistance to antibiotics due to changes in their:
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
Subtopic:  Cloning Vector |
 85%
From NCERT
NEET - 2022
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