Q. No.Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
Subtopic: Â Separation and Isolation of DNA fragments |
 92%
From NCERT
NEET - 2023
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Which one of the following statement is not true regarding gel electrophoresis technique?
| 1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
| 2. | The process of extraction of separated DNA strands from gel is called elution. |
| 3. | The separated DNA fragments are stained by using ethidium bromide. |
| 4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
Subtopic: Â Separation and Isolation of DNA fragments |
 84%
From NCERT
NEET - 2022
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Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic: Â Separation and Isolation of DNA fragments |
 90%
From NCERT
NEET - 2022
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NEET 2023 - Target Batch - Aryan Raj Singh
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Refer to the following statements for agarose-gel electrophoresis:
Choose the most appropriate answer from the options given below :
| (a) | Agarose is a natural polymer obtained from sea-weed. |
| (b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
| (c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
| (d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
Choose the most appropriate answer from the options given below :
| 1. | (a) and (b) only | 2. | (a), (b) and (c) only |
| 3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
Subtopic: Â Separation and Isolation of DNA fragments |
 82%
From NCERT
NEET - 2022
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DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
| 1. | Dark red bands | 2. | Bright blue bands |
| 3. | Yellow bands | 4. | Bright orange bands |
Subtopic: Â Separation and Isolation of DNA fragments |
 90%
From NCERT
NEET - 2021
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Histones | 2. | Polysaccharides |
| 3. | RNA | 4. | DNA |
Subtopic: Â Separation and Isolation of DNA fragments |Â General Design of an rDNA experiment |
 86%
From NCERT
NEET - 2021
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NEET 2023 - Target Batch - Aryan Raj Singh
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In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
Subtopic: Â Separation and Isolation of DNA fragments |
 92%
From NCERT
NEET - 2020
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Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
Subtopic: Â Polymerase Chain Reaction: PCR |Â Bioreactors/Downstream Processing |Â Separation and Isolation of DNA fragments |
 86%
From NCERT
NEET - 2020
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NEET 2023 - Target Batch - Aryan Raj Singh
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In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
Subtopic: Â Separation and Isolation of DNA fragments |
 91%
From NCERT
NEET - 2020
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Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
Subtopic: Â Process of Biotech |Â Separation and Isolation of DNA fragments |
 71%
From NCERT
NEET - 2020
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NEET 2023 - Target Batch - Aryan Raj Singh
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