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Q. No.Which of the following are correct about EcoR1?
A.
Cut the DNA with blunt end
B.
Cut the DNA with sticky end
C.
Recognises a specific palindromic sequence.
D.
Cut the DNA between the base G and A where encounters the DNA sequence 'GAATTC'
E.
Exonuclease
Choose the correct answer from the options given below:
1. B,C,E only
2. A,D,E only
3. A,C,D only
4. B,C,D only
Subtopic: Restriction Enzymes - Main Enzymes |
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Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
Subtopic: Polymerase Chain Reaction: PCR |
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Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:
1. Hind II, Hind II
2. Hind II, Alu I
3. Hind II, EcoR I
4. Hind II, Bam HI
1. Hind II, Hind II
2. Hind II, Alu I
3. Hind II, EcoR I
4. Hind II, Bam HI
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
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Identify the incorrect statement related to electrophoresis:
| 1. | Separated DNA fragments can be directly seen under UV radiation |
| 2. | Separated DNA can be extracted from gel piece. |
| 3. | Fragments of DNA moves toward anode. |
| 4. | Sieving effect of agarose gel helps in separation of DNA fragments. |
Subtopic: Separation and Isolation of DNA fragments |
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In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:
1. recognition sequence
2. cloning site
3. restriction site
4. ori site
1. recognition sequence
2. cloning site
3. restriction site
4. ori site
Subtopic: Principles of Biotechnology |
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Given below are two statements:
In light of the above statements, choose the correct answer from the options given below:
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
| Statement I: | Restriction Endonuclease finds its specific recognition sequence and binds to the DNA. |
| Statement II: | Restriction Endonuclease cuts each of the two strands of the double helix at specific points in their sugar phosphate backbones. |
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
Subtopic: Restriction Enzymes - Main Enzymes |
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Match List-I with List-II
Choose the correct answer from the options given below:
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
| List-I | List-II | ||
| A. | Ampicillin | I. | Restriction sites |
| B. | Ori | II. | Blue/White screening |
| C. | Multiple cloning sites | III. | Selectable marker |
| D. | \(\beta\) galactosidase gene | IV. | Sequence from where replication starts |
Choose the correct answer from the options given below:
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
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