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Q. No.Which of the following statement is incorrect above Agrobacterium tumefaciens ?
1.
It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells.
2.
'Ti' plasmid from Agrobacterium tumefaciens used for gene transfer is not pathogenic to plant cells.
3.
It transforms normal plant cells into tumor cells
4.
It delivers 'T-DNA' into plant cell
Subtopic: Transforming Plant & Animal Cell |
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Match List - I with List - II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
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Which of the following can act as molecular scissors?
1. Restriction enzymes
2. DNA ligase
3. RNA polymerase
4. DNA polymerase
1. Restriction enzymes
2. DNA ligase
3. RNA polymerase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
1. PsT I
2. Pvu I
3. EcoR I
4. BamH I
1. PsT I
2. Pvu I
3. EcoR I
4. BamH I
Subtopic: Cloning Vector |
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Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
Subtopic: Polymerase Chain Reaction: PCR |
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
Choose the correct answer from the options given below:
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Process of Biotech |
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In gene gun method used to introduce alien DNA into host cells, microparticles of ___________ metal are used.
1. Silver
2. Copper
3. Zinc
4. Tungsten or gold
1. Silver
2. Copper
3. Zinc
4. Tungsten or gold
Subtopic: Transforming Plant & Animal Cell |
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Polysaccharides | 2. | RNA |
| 3. | DNA | 4. | Histones |
Subtopic: General Design of an rDNA experiment |
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Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
Subtopic: Separation and Isolation of DNA fragments |
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Refer to the following statements for agarose-gel electrophoresis:
Choose the most appropriate answer from the options given below :
| (a) | Agarose is a natural polymer obtained from sea-weed. |
| (b) | The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. |
| (c) | The DNA migrates from negatively-charged electrode to the positively-charged electrode. |
| (d) | The DNA migrates from positively-charged electrode to the negatively-charged electrode. |
Choose the most appropriate answer from the options given below :
| 1. | (a) and (b) only | 2. | (a), (b) and (c) only |
| 3. | (a), (b) and (d) only | 4. | (b), (c) and (d) only |
Subtopic: Separation and Isolation of DNA fragments |
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