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Q. No.Refer to the following statements for agarose-gel electrophoresis:
(a)
Agarose is a natural polymer obtained from sea-weed.
(b)
The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA.
(c)
The DNA migrates from negatively-charged electrode to the positively-charged electrode.
(d)
The DNA migrates from positively-charged electrode to the negatively-charged electrode.
Choose the most appropriate answer from the options given below :
1.
(a) and (b) only
2.
(a), (b) and (c) only
3.
(a), (b) and (d) only
4.
(b), (c) and (d) only
Subtopic: Separation and Isolation of DNA fragments |
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Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?
1. Agrobacterium mediated transformation
2. Gene gun
3. 'Disarmed pathogen' vectors
4. Bacteriophages
1. Agrobacterium mediated transformation
2. Gene gun
3. 'Disarmed pathogen' vectors
4. Bacteriophages
Subtopic: Transforming Plant & Animal Cell |
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Pathogenic bacteria gain resistance to antibiotics due to changes in their :
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
Subtopic: Cloning Vector |
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The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
Subtopic: General Design of an rDNA experiment |
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Match List-I with List-II :
Choose the correct answer from the options given below:
| List I | List II | ||
| (a) | Gene gun | (i) | Replacement of a faulty gene by a normal healthy gene |
| (b) | Gene therapy | (ii) | Used for transfer of gene |
| (c) | Gene cloning | (iii) | Total DNA in the cells of an organism |
| (d) | Genome | (iv) | To obtain identical copies of a particular DNA molecule |
Choose the correct answer from the options given below:
| (a) | (b) | (c) | (d) | |
| 1. | (ii) | (i) | (iv) | (iii) |
| 2. | (i) | (iii) | (ii) | (iv) |
| 3. | (iv) | (i) | (iii) | (ii) |
| 4. | (ii) | (iii) | (iv) | (i) |
Subtopic: Transforming Plant & Animal Cell |
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Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic: Separation and Isolation of DNA fragments |
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Given below are two statements one is labelled as Assertion (A) and the other is labelled as Reason (R).
In light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
| Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
In light of the above statements, choose the correct answer from the options given below:
| 1. | Both (A) and (R) are true and (R) correctly explains (A). |
| 2. | Both (A) and (R) are true and (R) does not correctly explain (A). |
| 3. | (A) is true, (R) is false. |
| 4. | (A) is false, (R) is true. |
Subtopic: Restriction Enzymes - Main Enzymes |
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Cloning Vector | Tools |
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In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Subtopic: Polymerase Chain Reaction: PCR |
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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
| 1. | Escherichia coli | 2. | Bacillus thuringiensis |
| 3. | Salmonella typhimurium | 4. | Agrobacterium tumefaciens |
Subtopic: Process of Biotech | Introduction & History |
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