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Q. No.Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R)
Assertion (A):
Polymerase chain reaction is used in DNA amplification.
Reason (R):
The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements, choose the correct answer from the options given below:
1.
(A) is not correct but (R) is correct.
2.
Both (A) and (R) are correct and (R) is the correct explanation of (A).
3.
Both (A) and (R) are correct but (R) is not the correct explanation of (A).
4.
(A) is correct but (R) is not correct.
Subtopic: Selectable Markers: I | Polymerase Chain Reaction: PCR |
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Which one of the following statement is not true regarding gel electrophoresis technique?
| 1. | Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light. |
| 2. | The process of extraction of separated DNA strands from gel is called elution. |
| 3. | The separated DNA fragments are stained by using ethidium bromide. |
| 4. | The presence of chromogenic substrate gives blue coloured DNA bands on the gel. |
Subtopic: Separation and Isolation of DNA fragments |
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In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?
1. 5'GTATTC3' ; 3'CATAAG5'
2. 5'GATACT3' ; 3'CTATGA5'
3. 5'GAATTC3' ; 3'CTTAAG5'
4. 5'CTCAGT3' ; 3'GAGTCA5'
Subtopic: Restriction Enzymes - Main Enzymes |
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Give below are two statements:
In the light of the above statements, choose the most appropriate answer from the options given below:
1. Statement I is incorrect but Statement II is correct.
2. Both Statement I and Statement II are correct.
3. Both Statement I and Statement II are incorrect.
4. Statement I is correct but Statement II is incorrect.
| Statement I: | Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. |
| Statement II: | Restriction endonucleases cut the DNA strand a little away from the centre of the Palindromic site. |
In the light of the above statements, choose the most appropriate answer from the options given below:
1. Statement I is incorrect but Statement II is correct.
2. Both Statement I and Statement II are correct.
3. Both Statement I and Statement II are incorrect.
4. Statement I is correct but Statement II is incorrect.
Subtopic: Restriction Enzymes - Main Enzymes |
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Which of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Subtopic: Cloning Vector |
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Given below are two statements: One is labeled as Assertion (A) and the other is labeled as Reason (R)
| Assertion (A): | Restriction enzyme is a type of endonuclease |
| Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is correct but (R) is not correct. |
| 2. | (A) is not correct but (R) is correct. |
| 3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Subtopic: Restriction Enzymes - Main Enzymes |
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Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology non-recombinants transformed bacteria grow on the medium containing ampicillin as well as medium containing tetracycline |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is correct but (R) is not correct. |
| 2. | (A) is not correct but (R) is correct. |
| 3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Subtopic: General Design of an rDNA experiment |
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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
| 1. | Escherichia coli | 2. | Bacillus thuringiensis |
| 3. | Salmonella typhimurium | 4. | Agrobacterium tumefaciens |
Subtopic: Process of Biotech | Introduction & History |
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In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Subtopic: Polymerase Chain Reaction: PCR |
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Cloning Vector | Tools |
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