Q. No.A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
1. Palindromic Nucleotide sequences
2. Poly(A) tail sequences
3. Degenerate primer sequence
4. Okazaki sequences
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
| 1. | Denaturation | 2. | Ligation |
| 3. | Annealing | 4. | Extension |
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
| 1. | it will lead to the lysis of host cells. |
| 2. | it will be able to produce a novel protein with dual abilities. |
| 3. | it will not be able to confer ampicillin resistance to the host cell. |
| 4. | the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside. |
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Histones | 2. | Polysaccharides |
| 3. | RNA | 4. | DNA |
DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:
| 1. | Dark red bands | 2. | Bright blue bands |
| 3. | Yellow bands | 4. | Bright orange bands |
Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
In recombinant DNA technology antibiotics are used:
1. to keep medium bacteria-free
2. to detect alien DNA
3. to impart disease-resistance to the host plant
4. as selectable markers
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