Q. No.In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
Identify the wrong statement with regards to restriction enzyme:
| 1. | They cut the strength at palindromic sites. |
| 2. | They are useful in genetic engineering. |
| 3. | Sticky ends can be joined by using DNA ligases. |
| 4. | Each restriction enzyme functions by inspecting the length of a DNA sequence. |
The specific palindromic sequence which is recognized by EcoRI is:
| 1. | 5' -GGAACC-3' 3'-CCTTGG-5' |
2. | 5'-CTTAAG-3' 3'-GAATTC-5' |
| 3. | 5'-GGATCC-3' 3'-CCTAGG-5' |
4. | 5'-GAATTC-3' 3'-CTTAAG-5' |
2. Palindromic sequence
3. Recognition site
4. Selectable marker
First discovered restriction endonuclease that always cuts DNA molecules at a particular point by recognizing a specific sequence of six base pairs is:
1. EcoRI
2. Adenosine deaminase
3. Thermostable DNA polymerase
4. Hind II
In recombinant DNA technology antibiotics are used:
1. to keep medium bacteria-free
2. to detect alien DNA
3. to impart disease-resistance to the host plant
4. as selectable markers
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
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