Q. No.An enzyme catalysing the removal of nucleotides from ends of DNA is:
1. DNA ligase
2. Endonuclease
3. Exonuclease
4. Protease
1. DNA ligase
2. Endonuclease
3. Exonuclease
4. Protease
Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:
| (a) | DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal. |
| (b) | DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA. |
| (c) | The smaller the DNA fragment, the greater the distance it travels through it. |
| (d) | Pure DNA can be visualized directly by exposing it to UV radiation. |
Choose the correct answer from the options given below:
| 1. | (a), (c) and (d) | 2. | (a), (b) and (c) |
| 3. | (b), (c) and (d) | 4. | (a), (b) and (d) |
A selectable marker is used to:
| 1. | help in eliminating the non-transformants so that the transformants can be regenerated. |
| 2. | identify the gene for the desired trait in an alien organism. |
| 3. | select a suitable vector for transformation in a specific crop. |
| 4. | mark a gene on a chromosome for isolation using a restriction enzyme. |
The two antibiotic resistance genes on vector pBR 322 are for:
1. Ampicillin and Tetracycline
2. Ampicillin and Chloramphenicol
3. Chloramphenicol and Tetracycline
4. Tetracycline and Kanamycin
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | (iii) | (i) | (iv) | (ii) |
| 2. | (iii) | (iv) | (i) | (ii) |
| 3. | (iv) | (iii) | (i) | (ii) |
| 4. | (ii) | (iv) | (i) | (iii) |
The correct order of steps in Polymerase Chain Reation (PCR) is
(1) Extension Denaturation, Annealing
(2) Annealing, Extension, Denaturation
(3) Denaturation, Annealing, Extension
(4) Denaturation, Extension, Annealing
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
| 1. | Bioreactor | 2. | BOD incubator |
| 3. | Sludge digester | 4. | Industrial oven |
DNA precipitation, out of a mixture of biomolecules, can be achieved by treatment with:
1. Chilled chloroform
2. Isopropanol
3. Chilled ethanol
4. Methanol at room temperature
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement:
| 1. | The enzyme recognizes a specific palindromic nucleotide sequence in the DNA |
| 2. | The enzyme cuts DNA molecules at an identified position within the DNA |
| 3. | The enzyme binds DNA at specific sites and cuts only one of the two strands |
| 4. | The enzyme cuts the sugar-phosphate backbone at specific sites on each strand |
2. Palindromic sequence
3. Recognition site
4. Selectable marker
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