'Insertional inactivation' is used for
1. | Selection of recombinants |
2. | Producing pest resistant crops |
3. | Enhancing nutritional value of food |
4. | Reducing post harvest losses |
Selection of recombinants is based on all except one. Find out the exception
1. | Expression and non-expression of genes encoding for tetracycline-resistant compound |
2. | Expression and non-expression of genes encoding for insulin-resistant compound |
3. | Expression and non-expression of genes encoding for ampicillin-resistant compound |
4. | Insertional inactivation |
When recombinant DNA is inserted within the coding sequence of an enzyme β-galactosidase. This results in the following except
1. | Insertional inactivation |
2. | Recombinant colonies do not produce any color |
3. | Inactivation of enzyme |
4. | Chromogenic substrate is converted into product by recombinant colonies |
If a foreign gene is inserted into pBR322 by using restriction enzyme BamHI, the selection process, for identifying recombinants requires plating on all except
1. | Ampicillin rich medium |
2. | Tetracycline rich medium |
3. | X-gal rich medium |
4. | Nutrient medium without antibiotics |
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
1. | Non-recombinant bacteria containing -galactosidase |
2. | Insertional inactivation of -galactosidase in non-recombinant bacteria |
3. | Insertional inactivation of β-galactosidase in recombinant bacteria |
4. | Inactivation of glycosidase enzyme in recombinant bacteria |