List I | List II | ||
A. | Genetically engineered Human Insulin | I. | Gene therapy |
B. | GM Cotton | II. | E.Coli |
C. | ADA Deficiency | III. | Antigen-antibody interaction |
D. | ELISA | IV. | Bacillus thuringiensis |
1. | Enzyme Linked Immuno-Sorbent Assay (ELISA) technique |
2. | Recombinant DNA Technology |
3. | Serum and Urine analysis |
4. | Polymerase Chain Reaction (PCR) technique |
List-I | List-II | ||
(A) | Gene therapy | (I) | Separation of DNA fragments |
(B) | RNA interference | (II) | Diagnostic test for AIDS |
(C) | ELISA | (III) | Cellular defense |
(D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
Options: | (A) | (B) | (C) | (D) |
1. | IV | I | II | III |
2. | IV | II | III | I |
3. | IV | III | II | I |
4. | IV | III | I | II |
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
1. Purification of isolated protein
2. Detection of gene mutation
3. Molecular diagnosis
4. Gene amplification
Nowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
1. | mutated gene does not appear on a photographic film as the probe has no complementarity with it. |
2. | mutated gene does not appear on a photographic film as the probe has complementarity with it. |
3. | mutated gene partially appears on a photographic film. |
4. | mutated gene completely and clearly appears on a photographic film. |
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
1. | ELISA Technique | 2. | Hybridization Technique |
3. | Western Blotting Technique | 4. | Southern Blotting Technique |
Match the following columns and select the correct option.
Column-I | Column-II | ||
a. | Bt cotton | (i) | Gene therapy |
b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
c. | RNAi | (iii) | Detection of HIV infection |
d. | PCR | (iv) | Bacillus thuringiensis |
a | b | c | d | |
1. | (iii) | (ii) | (i) | (iv) |
2. | (ii) | (iii) | (iv) | (i) |
3. | (i) | (ii) | (iii) | (iv) |
4. | (iv) | (i) | (ii) | (iii) |