Statement I: | Two DNA sequences were prepared corresponding to A and B, chains of human insulin and were introduced in the plasmids of Agrobacterium to produce insulin chain. |
Statement II: | Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin. |
List I | List II | ||
A. | Genetically engineered Human Insulin | I. | Gene therapy |
B. | GM Cotton | II. | E.Coli |
C. | ADA Deficiency | III. | Antigen-antibody interaction |
D. | ELISA | IV. | Bacillus thuringiensis |
1. | Mature insulin molecule consists of three polypeptide chains - A, B and C. |
2. | Insulin is synthesized as prohormone which contains extra stretch of C-peptide. |
3. | C-peptide is not present in mature insulin molecule. |
4. | Polypeptide chains A and B are linked to disulphide bridges. |
List-I | List-II | ||
(A) | Gene therapy | (I) | Separation of DNA fragments |
(B) | RNA interference | (II) | Diagnostic test for AIDS |
(C) | ELISA | (III) | Cellular defense |
(D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
Options: | (A) | (B) | (C) | (D) |
1. | IV | I | II | III |
2. | IV | II | III | I |
3. | IV | III | II | I |
4. | IV | III | I | II |
(a) | Pro-hormone insulin contain extra stretch of C-peptide |
(b) | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
(c) | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
(d) | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
(e) | Some patients develop allergic reactions to the foreign insulin. |
Genetically engineered insulin for human is produced from:
1. Escherichia coli
2. Psuedomonas putida
3. Bacillus thuringiensis
4. Rhizobium meliloti
Give below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): | In human beings, insulin is synthesized as a pro-hormone which needs to be processed before it becomes fully mature and functional. |
Reason (R): | The extra stretch of C-peptide is to be removed from A and B peptide chain of insulin. |
In the light of the above statements, Choose the most appropriate answer from the option given below :
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
With regard to insulin, choose the correct options:
a: | C-peptide is not present in mature insulin. |
b: | The insulin produced by rDNA technology has C-peptide. |
c: | The pro-insulin has C-peptide. |
d: | A-peptide and B-peptide of insulin are interconnected by disulphide bridges. |
1. | (a), (c) and (d) only | 2. | (a) and (d) Only |
3. | (b) and (d) only | 4. | (b) and (c) only |
Which of the following statements is not correct?
1. | The proinsulin has an extra peptide called C-peptide |
2. | Functional insulin has A and B chains linked together by hydrogen bonds. |
3. | Genetically engineered insulin is produced in E. Coli. |
4. | In man, insulin is synthesized as proinsulin. |