Statement I: | RNA interference takes place in all eukaryotic organisms as method of cellular defense. |
Statement II: | RNAi involves the silencing of a specific mRNA due to a complementary single stranded RNA molecule that binds and prevents translation of mRNA |
List-I | List-II | ||
(A) | Gene therapy | (I) | Separation of DNA fragments |
(B) | RNA interference | (II) | Diagnostic test for AIDS |
(C) | ELISA | (III) | Cellular defense |
(D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
Options: | (A) | (B) | (C) | (D) |
1. | IV | I | II | III |
2. | IV | II | III | I |
3. | IV | III | II | I |
4. | IV | III | I | II |
ds RNA can be used during which one of the following ?
1. Gene sequencing
2. Polymerase Chain Reaction
3. Gene silencing
4. Autoradiography
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
1. | (A) is correct but (R) is not correct. |
2. | (A) is not correct but (R) is correct. |
3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Match the organism with its use in biotechnology.
(a) | Bacillus thuringiensis | (i) | Cloning vector |
(b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
(c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
(d) | Salmonella typhimurium | (iv) | Cry proteins |
Select the correct option from the following:
(a) | (b) | (c) | (d) | |
1. | (iv) | (iii) | (i) | (ii) |
2. | (iii) | (ii) | (iv) | (i) |
3. | (iii) | (iv) | (i) | (ii) |
4. | (ii) | (iv) | (iii) | (i) |
Match the following columns and select the correct option.
Column-I | Column-II | ||
a. | Bt cotton | (i) | Gene therapy |
b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
c. | RNAi | (iii) | Detection of HIV infection |
d. | PCR | (iv) | Bacillus thuringiensis |
a | b | c | d | |
1. | (iii) | (ii) | (i) | (iv) |
2. | (ii) | (iii) | (iv) | (i) |
3. | (i) | (ii) | (iii) | (iv) |
4. | (iv) | (i) | (ii) | (iii) |
RNA interference is used for which of the following purposes in the field of biotechnology?
1. | to develop a plant tolerant to abiotic stresses |
2. | to develop a pest-resistant plant against infestation by nematode |
3. | to enhance the mineral usage by the plant |
4. | to reduce post-harvest losses |
In RNAi, the genes are silenced using:
1. | dsRNA | 2. | ssDNA |
3. | ssRNA | 4. | dsDNA |