| Statement I: | RNA interference takes place in all eukaryotic organisms as method of cellular defense. |
| Statement II: | RNAi involves the silencing of a specific mRNA due to a complementary single stranded RNA molecule that binds and prevents translation of mRNA |
| List-I | List-II | ||
| (A) | Gene therapy | (I) | Separation of DNA fragments |
| (B) | RNA interference | (II) | Diagnostic test for AIDS |
| (C) | ELISA | (III) | Cellular defense |
| (D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
| Options: | (A) | (B) | (C) | (D) |
| 1. | IV | I | II | III |
| 2. | IV | II | III | I |
| 3. | IV | III | II | I |
| 4. | IV | III | I | II |
ds RNA can be used during which one of the following ?
1. Gene sequencing
2. Polymerase Chain Reaction
3. Gene silencing
4. Autoradiography
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
| Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
| Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
| 1. | (A) is correct but (R) is not correct. |
| 2. | (A) is not correct but (R) is correct. |
| 3. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
Select the correct option from the following:
| (a) | (b) | (c) | (d) | |
| 1. | (iv) | (iii) | (i) | (ii) |
| 2. | (iii) | (ii) | (iv) | (i) |
| 3. | (iii) | (iv) | (i) | (ii) |
| 4. | (ii) | (iv) | (iii) | (i) |
Match the following columns and select the correct option.
| Column-I | Column-II | ||
| a. | Bt cotton | (i) | Gene therapy |
| b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
| c. | RNAi | (iii) | Detection of HIV infection |
| d. | PCR | (iv) | Bacillus thuringiensis |
| a | b | c | d | |
| 1. | (iii) | (ii) | (i) | (iv) |
| 2. | (ii) | (iii) | (iv) | (i) |
| 3. | (i) | (ii) | (iii) | (iv) |
| 4. | (iv) | (i) | (ii) | (iii) |
RNA interference is used for which of the following purposes in the field of biotechnology?
| 1. | to develop a plant tolerant to abiotic stresses |
| 2. | to develop a pest-resistant plant against infestation by nematode |
| 3. | to enhance the mineral usage by the plant |
| 4. | to reduce post-harvest losses |
In RNAi, the genes are silenced using:
| 1. | dsRNA | 2. | ssDNA |
| 3. | ssRNA | 4. | dsDNA |