Statement I: | Bt toxins are insect group specific and coded by a gene cry IAc. |
Statement II: | Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect, the inactive protoxin gets converted into active form due to acidic pH of the insect gut. |
List I | List II | ||
A. | \(\alpha-1\) antitrypsin | I. | Cotton bollworm |
B. | Cry IAb | II. | ADA deficiency |
C. | Cry IAc | III. | Emphysema |
D. | Enzyme replacement therapy | IV. | Corn borer |
1. | Bt toxin requires acidic pH for activation |
2. | Bacillus has protective mechanism against it |
3. | In Bacillus, it is present in an inactive state and once insects ingests it, it is converted to active form |
4. | The protoxin requires protein conjugate for its activation |
A. | The inactive toxin converted into active form due to alkaline pH of gut of insect |
B. | Bacillus thuringiensis produce crystals with toxic insecticidal proteins. |
C. | The alkaline pH solubilises the crystals. |
D. | The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect. |
E. | The toxin proteins exist as inactive protoxins in bacteria. |
List I | List II | ||
A. | Genetically engineered Human Insulin | I. | Gene therapy |
B. | GM Cotton | II. | E.Coli |
C. | ADA Deficiency | III. | Antigen-antibody interaction |
D. | ELISA | IV. | Bacillus thuringiensis |
Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (BT) is resistant to:
Match the organism with its use in biotechnology.
(a) | Bacillus thuringiensis | (i) | Cloning vector |
(b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
(c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
(d) | Salmonella typhimurium | (iv) | Cry proteins |
Select the correct option from the following:
(a) | (b) | (c) | (d) | |
1. | (iv) | (iii) | (i) | (ii) |
2. | (iii) | (ii) | (iv) | (i) |
3. | (iii) | (iv) | (i) | (ii) |
4. | (ii) | (iv) | (iii) | (i) |
Match the following columns and select the correct option.
Column-I | Column-II | ||
a. | Bt cotton | (i) | Gene therapy |
b. | Adenosine deaminase deficiency | (ii) | Cellular defense |
c. | RNAi | (iii) | Detection of HIV infection |
d. | PCR | (iv) | Bacillus thuringiensis |
a | b | c | d | |
1. | (iii) | (ii) | (i) | (iv) |
2. | (ii) | (iii) | (iv) | (i) |
3. | (i) | (ii) | (iii) | (iv) |
4. | (iv) | (i) | (ii) | (iii) |
What triggers the activation of protoxin to active Bt toxin of Bacillus thuringiensis in bollworms?
1. Acidic pH of the stomach
2. Body temperature
3. Moist surface of midgut
4. Alkaline pH of the gut