In HGP, the fragments of DNA were sequenced using automated DNA sequencers. It worked on the principle of a method developed by

1. E.M. Southern

2. Frederick Sanger

3. Craig Venter

4. Wellcome Trust (U.K.)

Which of the following is not correct with respect to DNA replication?

1. Eukaryotes have several thousand origins of replication

2. Unwinding is brought about by enzyme helicase, which is ATP dependent

3. DNA synthesis occurs in 3'$\to$5' direction

4. DNA polymerase-I removes RNA primer and replace it with the nucelotide of DNA and can correct the T=T dimer.

Which of the following is not a codon of glycine?

1. GGC

2. GCG

3. GGU

4. GGA

Which of the given is not among the salient features of HGP?

1. Human genome contains about 316.7 bp

2. Average gene size is 3000 bases

3. Less than 2% of genome codes for proteins

4. Chromosome 1 has most genes i.e., 2968

Transduction experiment to prove DNA as a genetic material was conducted by Hershey and Chase with the use of radioactive elements, namely

1. S³² and P³⁵ 

2. S³⁵ and P³² 

3. N¹⁵ and H³ 

4. C¹⁴ and H³ 

UTRs are required for efficient translation, which are present on

1. 3' mRNA

2. 5' mRNA

3. T$\mathrm{\psi }$C region of tRNA

4. Both (1) & (2)

During DNA finger printing experiment DNA bands of different sizes gives a characteristic pattern for an individual, called as

1. Dendrograph 

2. Autoradiogram

3. Cladogram

4. Phenogram

DNA polymorphism is equivalent to allelic sequence variation and 

a. These are inheritable

b. These can be observed in non coding DNA

c. Satellite DNA is an example for such variations

1. All are correct

2. Only c is correct

3. Both a and c are incorrect

4. Only b is incorrect

Which one of the following is incorrectly matched pair with respect to DNA fingerprinting?

1. Size separation - Gel electrophoresis

2. VNTRs from Gel to Nylon paper - Blotting technique

3. Microsatellites - ESTs

4. RFLP amplification - PCR

Choose the odd one out with respect to Taylor's experiment

1. Used roots of Vicia faba

2. Utilised radioactive thymidine

3. Used heavy nitrogen

4. Proved DNA in chromosomes replicate semi-conservatively