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Q. No.Identify the incorrectly matched pair
| Organism | Length of DNA | |
| 1. | φ ×174 | 5386 base pairs |
| 2. | Bacteriophage lambda | 48502 base pairs |
| 3. | Escherichia coli | 4.6 × 106 base pairs |
| 4. | Haploid content of human DNA | 3.3 × 109 base pairs |
Subtopic: The DNA |
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In a typical human cell:
| I: | the total number of bp in DNA present is about 6.6 × 109 |
| II: | the total length of unwinded DNA is approximately 2.2 metres |
| III: | the dimension of nucleus is approximately 10–6 m |
| 1. | Only I and II are correct |
| 2. | Only II and III are correct |
| 3. | I, II and III are correct |
| 4. | I, II, and III are incorrect |
Subtopic: DNA Packaging |
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A hypothetical dsDNA of 100 bp length molecule was found to be composed of 40 Adenine residues. The total number of hydrogen bonds in all base pairs of this molecule is expected to be:
| 1. | 100 | 2. | 130 |
| 3. | 260 | 4. | 300 |
Subtopic: DNA Double Helix |
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The molecule shown in the given figure:
| I: | serve as substrate for DNA replication |
| II: | provide energy for polymerisation reaction |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: DNA Replication: I |
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Normally a transcription unit will include all of the following except:
| 1. | Operator | 2. | Promoter |
| 3. | Structural gene | 4. | Terminator |
Subtopic: Transcription |
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Consider the given two statements:
| Assertion (A): | Euchromatin is said to be transcriptionally inactive chromatin. |
| Reason (R): | In a typical nucleus, euchromatin is the region of chromatin that is loosely packed and stains light. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is False but (R) is True. |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: DNA Packaging |
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The enzyme reverse transcriptase of Human Immunodeficiency Virus can be regarded as:
1. DNA dependent DNA polymerase
2. DNA dependent RNA polymerase
3. RNA dependent DNA polymerase
4. RNA dependent RNA polymerase
1. DNA dependent DNA polymerase
2. DNA dependent RNA polymerase
3. RNA dependent DNA polymerase
4. RNA dependent RNA polymerase
Subtopic: The DNA |
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The methodology used in human genome sequencing that took the blind approach of simply sequencing the
whole set of genome that contained all the coding and non-coding sequence, and later assigning different
regions in the sequence with functions, is called as:
1. Expressed sequence tag
2. Sequence annotation
3. Chain termination
4. Physical mapping
whole set of genome that contained all the coding and non-coding sequence, and later assigning different
regions in the sequence with functions, is called as:
1. Expressed sequence tag
2. Sequence annotation
3. Chain termination
4. Physical mapping
Subtopic: Human Genome Project |
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Which of the following statements is correct about the role of regulatory proteins in transcription in prokaryotes?
| 1. | They only increase the expression |
| 2. | They only decrease the expression |
| 3. | They interact with RNA polymerase but do not affect the expression |
| 4. | They can act both as activators and as repressors |
Subtopic: Gene Regulation: Lac Operon |
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In DNA fingerprinting involving Southern blot hybridisation using radiolabelled VNTR as a probe, the correct chronology of steps between isolation of DNA [first step] and detection of hybridised DNA fragments by autoradiography [last step] is:
| 1. | separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe |
| 2. | digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe |
| 3. | separation of DNA fragments by electrophoresis → digestion of DNA by restriction endonucleases → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon |
| 4. | digestion of DNA by restriction endonucleases →separation of DNA fragments by electrophoresis → hybridisation using labelled VNTR probe → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon |
Subtopic: DNA Fingerprinting |
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