1. |
The TCA cycle begins with the condensation of the acetyl group with oxaloacetic acid (OAA) to form citric acid, catalyzed by citrate synthase. |
2. |
The TCA cycle involves three steps where NAD⁺ is reduced to NADH + H⁺ and one step where FAD⁺ is reduced to FADH₂. |
3. |
During the conversion of succinyl-CoA to succinic acid, ATP is synthesized directly through substrate-level phosphorylation. |
4. |
The continuous functioning of the TCA cycle depends on the regeneration of oxaloacetic acid as well as NAD⁺ and FAD⁺ from NADH and FADH₂. |