NEET Zoology Biotechnology Principles and Processes Questions Solved


Recombinant DNA- technology is of great importance in the field of medicine. With

the help of a flow chart, show how this technology has been used in preparing genetically

engineered human insulins.                                                                               (3)




Insulin contains two polypeptide chains, A and B, linked by disulphide bridges


        Prepares two DNA sequences separately, Peptide A and Peptide B 


        Introduce them in plasmid of E.coli to produce insulin chains A and B 


         Extract peptide chains A and B from E.coil and combine them by creating 

                               disulphiede bond to form human insulin                                                         (3)


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