Why is making cells competent essential for biotechnology experiment? List any two ways by which this can be achieved. 2 mark
DNA is a hydrophilic molecule, it cannot pass through plasma membranes. In order to force bacteria to take up the plasmid, the bacterial cells must be made competent to take up DNA.
Two ways by which this can be achieved:
1. Biolistics or gene gun-cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.
2. Disarmed pathogen vectors, which when allowed to infect the cell, transfer the rDNA into the host.