NEET Zoology Biotechnology Principles and Processes Questions Solved

BOARD

A recombinant DNA is formed when sticky end of vector DNA and foreign DNA

join. Explain how the sticky ends are formed and get joined.

1. The restriction enzymes recognizes palindromic nucleotide sequence in the DNA 

at specific location. e.g. EcoR I or Hindi II.

2. It cuts the strands of DNA a little away from the center of palindrome sites, but 

between the same two bases on the opposite strands. This leaves single stranded 

portions at the ends, called sticky ends on each strand.

3. They can form hydrogen bonds with their complementary cut counterparts. This 

stickiness of the end facilitates the action of enzyme lipase.

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