A recombinant DNA is formed when sticky end of vector DNA and foreign DNA
join. Explain how the sticky ends are formed and get joined.
1. The restriction enzymes recognizes palindromic nucleotide sequence in the DNA
at specific location. e.g. EcoR I or Hindi II.
2. It cuts the strands of DNA a little away from the center of palindrome sites, but
between the same two bases on the opposite strands. This leaves single stranded
portions at the ends, called sticky ends on each strand.
3. They can form hydrogen bonds with their complementary cut counterparts. This
stickiness of the end facilitates the action of enzyme lipase.