NEET Zoology Biotechnology Principles and Processes Questions Solved

BOARD

Explain the importance of (a) ori, (b) ampR and (c) rop in the E.coli vector shown below:

(a) Ori-origin of replication: This is a sequence from where replication begins and any piece of DNA when linked to this sequence can be made to replicate within the host cells.

(b) ampR- antibiotic resistance gene (ampicillin). It acts as a selectable marker which helps in identifying and eliminating non transformants and selectively permitting the growth of the transformants.

(c) rop-code for the proteins involved in replication of the plasmid.

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