A major advantage of using YAC as a cloning vector over the plasmids is that :

  1. it can replicate independently
  2. it can be selected easily
  3. it can accomodate larger inserts
  4. it has multiple cloning sites

Concept Questions :-

Tools: Vectors:II
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An important limitation to the use of Agrobacterium tumefaciens is that it can not:

  1. infect dicots
  2. be genetically modified
  3. be cultured on a nutrient medium
  4. infect crop plants such as wheat and corn

Concept Questions :-

Tools: Vectors:II
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If a sample has a DNA of size 2kb and 6kb while the molecular marker denotes 4kb band as it's first band where will be the 6kb band of DNA?

A.      Above the band of another DNA sample

B.      Above the band of marker

C.      In between the band of marker and another band of DNA sample.

D.     Both A and B

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Which of the following is the reason of increased surface area for oxygen transfer in sparged bioreactor ?

A.      Bubbles

B.      Turbidity

C.      Cells

D.     Protein

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The formulation with suitable preservatives in the case of drugs has to undergo

A.      Through clinical trials

B.      Through strict separation techniques

C.      Through harsh heat shock control

D.     Through long restriction digestion

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Downstream processing is not

A.      Separation

B.      Purification

C.      Vary with product

D.     Occurs during marketing phase

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Quality control testing is not

A.      A part of downstream processing

B.      Include separation and purification

C.      Both A and B

D.     Vary from product to product

Concept Questions :-

Large Scale Production: II
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The Downstream processing occurs after

A.      Biosynthetic phase

B.      Separation phase

C.      Purification phase

D.     All of these

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Downstream processing includes

A.      Separation

B.      Purification

C.      Both A and B

D.     Quality control Testing

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Increased surface area for oxygen transfer is a feature found specifically in

A.      Simple stirred tank bioreactor

B.      Sparged stirred-tank bioreactor

C.      All stirred tank bioreactors

D.     All bioreactors have this property

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