Which methodology is used while sequencing the total DNA from a cell? Explain in it detail.
Methodology used: Sequence Annotation
Method of Sequence Annotation: Total DNA from a cell is isolated , converted into random fragments of relatively smaller sizes, and cloned in suitable host using specialized vectors.
The cloning results into amplification of each piece of DNA fragment. The fragments are sequenced using automated DNA sequencers, these sequences are then arranged based on some overlapping fragments (present in them).
This requires generation of overlapping fragments (for sequencing).
Specialized computer based programmes are developed , and these sequences are subsequently annotated and assigned to each chromosome.