# NEET Zoology Biotechnology Principles and Processes Questions Solved

BOARD

(a) In pBR322, foreign DNA has to be introduced in ${\mathrm{tet}}^{\mathrm{R}}$ region. From the restriction enzymes given below, which one should be used and why:

PvuI,EcoRI, BamHI

(b) Give reasons, why the other two enzymes cannot be used.

(a) BamHI should be used , as restriction site for this enzyme is present in ${\mathrm{tet}}^{\mathrm{R}}$ region.

(b) PvuI will not be used , as restriction site for this enzyme is present in ${\mathrm{amp}}^{\mathrm{R}}$ region and not in ${\mathrm{tet}}^{\mathrm{R}}$ region.

EcoRI will not be used , as restriction site for this enzyme is not present in selectable marker ${\mathrm{tet}}^{\mathrm{R}}$.

Difficulty Level:

• 95%
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