NEET Zoology Biotechnology Principles and Processes Questions Solved

BOARD

(a) In pBR322, foreign DNA has to be introduced in tetR region. From the restriction enzymes given below, which one should be used and why:

PvuI,EcoRI, BamHI

(b) Give reasons, why the other two enzymes cannot be used.

(a) BamHI should be used , as restriction site for this enzyme is present in tetR region.

(b) PvuI will not be used , as restriction site for this enzyme is present in ampR region and not in tetR region.

EcoRI will not be used , as restriction site for this enzyme is not present in selectable marker tetR.

Difficulty Level:

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