Mention the role of (i) selectable marker, (ii) Ori and (iii) rop in E. coli cloning vector pBR322.
i) Selectable marker - helps in identifying and eliminating non transformants and selectively permitting the growth of the transformants = 1
ii)Ori - helps to start replication and any piece of DNA when linked to this sequence can be made to replicate within host cell, responsible for controlling the copy number of the linked DNA = ½ + ½
iii)-codes for the proteins involved in the replication of
the plasmid = 1 3mark