The primers designed according to (Answer according to very basic PCR)
A. Terminal ends of vector
B. Terminal ends of gene of interest
C. Random primers
D. According to direction of PCR
Primers are not
B. Biologically synthesised
D. Complementary to the regions of DNA
Which of the following doesn't go with PCR?
A. Thermus aquaticus
B. 1 billion copies
C. Taq polymerase
If a strand has following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.
A. 5’TAACGGGATC 3’
B. 3’TAACGGGATC 5’
C. 5’ATTGCCCTAG 3’
D. 3’ATTGCCCTAG 5’
If a DNA strand has sequence 3’TAACGCTTGGCCAAGTCAGTCAG 5’
Design the primer for the strand has polarity 3’ to 5’
A. 5’ ATTGC 3’
B. 5’ TAAGC 3’
C. 3’ ATTGC 5’
D. 3’ TAAGC 5’
If a DNA strand has to undergo denaturation in PCR
A. Need to give optimal conditions
B. Temperature should be above 90 degree Celsius
C. Need to give Taq polymerase
D. A strand cannot undergo denaturation because of absence of hydrogen bonds
If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?
What is the final step of PCR?
After 30 cycle of PCR, how many times DNA will be amplified?
A. Thousand times
B. Million times
C. Billion times
D. Hundreds times
Use of primers, occurs in which step of PCR?
D. Both extension and Annealing