In a DNA gel the fragment of 2kb and 4kb will be

A.      2kb nearer to cathode while 4kb nearer to anode

B.      2kb closer to wells while 3kb towards the opposite ends

C.      2kb closer to opposite end of gel while 3kb closer to well

D.     2kb away from cathode while 3kb towards anode

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If we are able to link an alien piece of DNA with ……a…...We can multiply it's number equal to the copy number of

…a…..

A.      a- bacteriophage

B.      a- Plasmid

C.      a- bacteriophage or Plasmid

D.     a- prion

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Which of the following is not a feature to facilitate cloning into a vector?

A.      Origin of replication

B.      Selectable Marker

C.      Multiple cloning site

D.     Virulence region

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If I want to check the expression of my gene, then I should use

A.      Vectors supporting high copy number

B.      Vectors supporting low copy number

C.      No need to bother about copy number

D.     Vectors with strong Ori

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Selectable marker  helps

A.      To identify transformants

B.      To identify recombinants

C.      To identify non- recombinants

D.     To eliminate transformants

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The normal E. coli cells have

A.      Kanamycin

B.      Tetracycline

C.      Ampicillin

D.     No resistance

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For easiest cloning, one needs to get vector with

A.      Single cloning site

B.      Multiple cloning site

C.      Few cloning site

D.     No cloning site at all

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If the gene of Beta galactosidase is not inactivated, means there will be color in the colonies of

A.      Non- recombinant

B.      Recombinant

C.      Transformants

D.     Non-transformants

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Insertional inactivation results in

A.      Non-recombinants

B.      Recombinants

C.      Transformants

D.     Non- transformants

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Agrobacterium tumifaciens

A.      A pathogen of  angiosperms

B.      It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

C.      Ti plasmid of it is now modified into an expression vector

D.     It's Ti plasmid is modified into a vector which is no more pathogenic

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