An ideal plasmid for recombinant technology DNA technology  

  1. Has maximum amount of DNA
  2. Has relaxed replication control
  3. Many recognition sites for one RE
  4. All of these

Concept Questions :-

Tools: Vectors: I
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Electroporation facilitates insertion of foreign DNA into the cell by

1. Changing the porocity of the membrane

2. Changing the electrical potential of the membrane

3. Lysing the cell wall

4. Active transport

Concept Questions :-

Tools: Vectors:II
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Alec Jeffrey’s name is associated with

a. DNA sequencing

b. DNA fingerprinting

c. RNA sequencing

d. Gene cloning

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The names associated with construction of first Recombinant DNA are

a. Arber, Nathans, Smith

b. Annie Chang, Boyer, Berg, Cohen

c. Howard Temin, Brenner, Sharp

d. Tim Hunt, Hartwell, Nurse

Concept Questions :-

Tools: Enzymes:I
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The restriction enzymes most commonly used in rDNA technology are

1. Type 1 Restriction enzymes

2. Type II Restriction enzymes

3. Type III Restriction enzymes

4. Type IV Restriction enzymes

Concept Questions :-

Tools: Enzymes:II
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Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because
a. Host DNA is packed into chromosomes
b. Restriction enzymes are ineffective on host DNA
c. Host DNA does not have the restriction site for the Restriction enzymes.
d. Restriction site of host DNA is methylated.

Concept Questions :-

Tools: Enzymes:II
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A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?
a. Southern blotting and South Western blotting
b. Northern blotting and western blotting
c. Western blotting and Eastern blotting
d. Southern and Northern blotting

Concept Questions :-

Process of Biotech
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Insertional inactivation of a gene helps in
a. Identification of recombinant clones
b. Identification of deletion mutants
c. Identification of recombinant transformants
d. Elimination of suppression mutants

Concept Questions :-

Selectable Markers:III
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What is true about the DNA polymerase used in PCR?

  1. Isolated from a virus
  2. Stable at high temperatures
  3. Used as selectable marker
  4. Has methylated bases
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Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particle are made up of
a. Zinc or tungsten
b. Silicon or gold
c. Tungsten or gold
d. Selenium or Platinum

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