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Q. No.Restriction enzymes produced by some bacteria:
1.
cleave phosphodiester bond in the main chromosomal DNA of the bacterium.
2.
cut single stranded DNA at random positions.
3.
prevent the multiplication of bacteriophage in bacteria.
4.
provide resistance against antibiotics.
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
51%
Level 3: 35%-60%
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Which of the following methods is especially useful for transforming animal cells?
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
1. Electroporation
2. Biolistics
3. Microinjection
4. Heat shock
Subtopic: Transforming Plant & Animal Cell |
89%
Level 1: 80%+
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The DNA fragments separated on agar gel by electrophoresis can be visualised:
| 1. | without staining in the visible light |
| 2. | without staining on exposure to UV light |
| 3. | after staining with polyethylene glycol followed by exposure to IR radiation |
| 4. | after staining with ethidium bromide followed by exposure to UV radiation |
Subtopic: Separation and Isolation of DNA fragments |
94%
Level 1: 80%+
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Tetracycline resistance gene in the vector pBR322 carries the recognition sequence for:
1. PstI
2. PvuII
3. BamH I
4. EcoR I
1. PstI
2. PvuII
3. BamH I
4. EcoR I
Subtopic: Cloning Vector |
89%
Level 1: 80%+
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If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
Subtopic: Restriction Enzymes: Historical Background | Tools |
91%
Level 1: 80%+
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The number of times the recognition sequence of EcoRI is expected to appear in a dsDNA of 10,000 bp length will be:
| 1. | 1 | 2. | 2 |
| 3. | 4 | 4. | 8 |
Subtopic: Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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A plasmid has a single recognition sequence for EcoRI. When cut with this enzyme, the number of resultant fragments will be:
1. 1
2. 2
3. 3
4. 4
Subtopic: Cloning Vector |
Level 3: 35%-60%
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The most critical requirement for any DNA molecule to be able to act as a cloning vector during rDNA procedures will be:
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
Subtopic: Cloning Vector |
89%
Level 1: 80%+
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Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
Subtopic: Separation and Isolation of DNA fragments |
93%
Level 1: 80%+
NEET - 2023
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
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NEET 2026 - Target Batch - Vital
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Polysaccharides | 2. | RNA |
| 3. | DNA | 4. | Histones |
Subtopic: General Design of an rDNA experiment |
89%
Level 1: 80%+
NEET - 2023
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
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NEET 2026 - Target Batch - Vital
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