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Q. No.If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
Subtopic: Restriction Enzymes: Historical Background | Tools |
91%
Level 1: 80%+
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The number of times the recognition sequence of EcoRI is expected to appear in a dsDNA of 10,000 bp length will be:
| 1. | 1 | 2. | 2 |
| 3. | 4 | 4. | 8 |
Subtopic: Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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A plasmid has a single recognition sequence for EcoRI. When cut with this enzyme, the number of resultant fragments will be:
1. 1
2. 2
3. 3
4. 4
Subtopic: Cloning Vector |
Level 3: 35%-60%
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The most critical requirement for any DNA molecule to be able to act as a cloning vector during rDNA procedures will be:
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
Subtopic: Cloning Vector |
88%
Level 1: 80%+
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Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
Subtopic: Separation and Isolation of DNA fragments |
93%
Level 1: 80%+
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
| 1. | Polysaccharides | 2. | RNA |
| 3. | DNA | 4. | Histones |
Subtopic: General Design of an rDNA experiment |
89%
Level 1: 80%+
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In gene gun method used to introduce alien DNA into host cells, microparticles of ___________ metal are used.
| 1. | Silver | 2. | Copper |
| 3. | Zinc | 4. | Tungsten or gold |
Subtopic: Transforming Plant & Animal Cell |
93%
Level 1: 80%+
NEET - 2023
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Process of Biotech |
Level 3: 35%-60%
NEET - 2023
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Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
Subtopic: Polymerase Chain Reaction: PCR |
90%
Level 1: 80%+
NEET - 2023
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
| 1. | PsT I | 2. | Pvu I |
| 3. | EcoR I | 4. | BamH I |
Subtopic: Cloning Vector |
85%
Level 1: 80%+
NEET - 2023
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