In order to obtain virus-free plants through tissue culture the best method is:
(a) protoplast culture
(b) embryo culture
(c) anther culture
(d) meristem culture
(d) In meristem culture, the shoot apical meristem along with some surrounding tissue is grown in vitro. It is used for clonal propagation and recovery of virus-free plants. It is also potentially useful in germ plasma exchange and long-term storage of germ plasma through freeze preservation. Protoplast culture involves the culture of protoplasts (Plant cell without cell wall) on a suitable medium. Embryo rescue is the culture of the incised immature embryo to raise plants. Anther culture involves the culture of anthers on artificial culture medium. It is helpful in obtaining haploid plants.