PCR and Restriction Fragment Length Polymorphism are the methods for

(a) study of enzymes

(b) genetic transformation

(c) DNA sequencing

(d) genetic fingerprinting

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A single strand of nucleic acid tagged with a radioactive molecule is called

(a) vector

(b) selectable marker

(c) plasmid

(d) probe

 

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Which one of the following is a case of wrong matching ?

(a) Somatic hybridisation - Fusion of two diverse cells

(b) Vector DNA - Site for tRNA synthesis

(c) Micropropogation - In vitro production of plants in large numbers

(d) Callus - Unorganised mass of cells produced in tissue culture

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Which one is a one statement regarding DNA polymerase used in PCR?

(a) It is used to ligate introduced DNA in recipient cells

(b) It serves as a selectable marker

(c) It is isolated from a virus

(d) It remains active at high temperature

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For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of

(a) silver or platinum

(b) platinum or zinc

(c) silicon or platinum

(d) gold or tungsten

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Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it?

5'__ GAATTC_ 3'

3' __ CTTAAG__5'

(a) Deletion mutation

(b) Start codon at the 5' end

(c) Palindromic sequence of base pairs

(d) Replication completed

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There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?

(a) Coelom                         (b) Coenzyme

(c) coli                               (d) Colon

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Agarose extracted from sea weeds finds use in

(a) tissue culture

(b) PCR

(c) gel electrophoresis

(d) spectrophotometry

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Continuous addition of sugars in ‘fed batch’ fermentation is done to

(a) obtain antibiotics

(b) purify enzymes

(c) degrade sewage

(d) produce methane

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Which one of the following is used as vector for cloning genes into higher organisms?

(a) Baculovirus

(b) Salmonella typhimurium

(c) Rhizopus nigricans

(d) Retrovirus

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