NEET Zoology Biotechnology Principles and Processes Questions Solved

NEET - 2012

PCR and Restriction Fragment Length Polymorphism are the methods for

(a) study of enzymes

(b) genetic transformation

(c) DNA sequencing

(d) genetic fingerprinting

The hypervariable regions of DNA have Variable Number of Tandem repeats (VNTRs) of short

nucleotide sequences which are specific for each individual (except for monozygotic twins). Variations

also occur due to mutations, small deletions or insertions. Therefore, DNA cut by a particular

endonuclease will have different lengths in different persons. This phenomenon is called Restriction

Fragment Length Polymorphism. It is the basis of genetic (or DNA) fingerprinting and is useful in

identifying individuals from their semen, blood or tissues or any other DNA sample and resolution of

parent hood disputes. Polymerase Chain Reaction (PCR) is also useful in genetic fingerprinting as it can

amplify the DNA sample even if available in a very small amount.

Difficulty Level:

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