The DNA molecule to which the gene of interest is integrated for cloning is called:
1. | Transformer | 2. | Vector |
3. | Template | 4. | Carrier |
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
1. | centrifugation |
2. | polymerase chain reaction |
3. | electrophoresis |
4. | restriction mapping |
Which of the following is not correctly matched for the organism and its cell wall
degrading enzyme?
1. Bacteria —Lysozyme
2. Plant cells — Cellulase
3. Algae —Methylase
4. Fungi — Chitinase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
1. | Non-recombinant bacteria containing -galactosidase |
2. | Insertional inactivation of -galactosidase in non-recombinant bacteria |
3. | Insertional inactivation of β-galactosidase in recombinant bacteria |
4. | Inactivation of glycosidase enzyme in recombinant bacteria |
The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. rop-reduced osmotic pressure
3. Hind III, Eco RI -selectable markers
4. ampR, tetR-antibiotic resistance genes
PCR and Restriction Fragment Length Polymorphism are the methods for
1. study of enzymes
2. genetic transformation
3. DNA sequencing
4. genetic fingerprinting
A single strand of nucleic acid tagged with a radioactive molecule is called
1. vector
2. selectable marker
3. plasmid
4. probe
Which one of the following is a case of wrong matching ?
1. Somatic hybridisation - Fusion of two diverse cells
2. Vector DNA - Site for tRNA synthesis
3. Micropropogation - In vitro production of plants in large numbers
4. Callus - Unorganised mass of cells produced in tissue culture
Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduced DNA in recipient cells
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature