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Which of the following restriction enzymes produces blunt ends?

1. Sal I                           
2. Eco RV
3. Xho                           
4. Hind III

Subtopic:  Tools: Enzymes: II | Tools |
Level 3: 35%-60%
NEET - 2016
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Which of the following is a endonuclease?

(1) Protease

(2) DNase I

(3) RNase restriction

(4) Hind II

Subtopic:  Tools: Enzymes: II | Tools |
 84%
Level 1: 80%+
NEET - 2016
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The Taq polymerase enzyme is obtained from

1. Thiobacillus ferroxidans

2. Bacillus subtilis

3. Pseudomonas subtilis

4. Thermus aquaticus

Subtopic:  Polymerase Chain Reaction: PCR |
 94%
Level 1: 80%+
NEET - 2016
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The cutting of DNA at specific locations became possible with the discovery of 

(1) restriction enzymes

(2) probes

(3) selectable markets

(4) ligases

Subtopic:  Introduction & History: II | Tools: Enzymes: II | Introduction & History | Tools |
 94%
Level 1: 80%+
NEET - 2015
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The DNA molecule to which the gene of interest is integrated for cloning is called:

1. Transformer 2. Vector
3. Template 4. Carrier
Subtopic:  Tools: Vectors: I | Tools |
 85%
Level 1: 80%+
NEET - 2015
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Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Subtopic:  Tools: Vectors: I | Tools |
 76%
Level 2: 60%+
NEET - 2014
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

1. centrifugation
2. polymerase chain reaction
3. electrophoresis
4. restriction mapping
Subtopic:  Process of Biotech |
 87%
Level 1: 80%+
NEET - 2013
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Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

1. Bacteria —Lysozyme

2. Plant cells — Cellulase

3. Algae —Methylase

4. Fungi — Chitinase

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 92%
Level 1: 80%+
NEET - 2013
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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

1. Non-recombinant bacteria containing β-galactosidase
2. Insertional inactivation of α-galactosidase in non-recombinant bacteria
3. Insertional inactivation of β-galactosidase in recombinant bacteria
4. Inactivation of glycosidase enzyme in recombinant bacteria

Subtopic:  Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
 70%
Level 2: 60%+
NEET - 2013
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The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?

1. Ori-original restriction enzyme

2. rop-reduced osmotic pressure

3. Hind III, Eco RI -selectable markers

4. ampR, tetR-antibiotic resistance genes

Subtopic:  Tools: Vectors: II | Cloning Vector | Tools |
 83%
Level 1: 80%+
NEET - 2012
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