Identify the properties of a good vector used in rDNA technology:
(a)
It should have origin of replication supporting a high copy number
(b)
It should have preferably more than '2' recognition sites
(c)
The restriction sites in vector should be in the antibiotic- resistant genes
(d)
It should have suitable marker genes
(e)
It should be easy to isolate and purify
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) only
2. (c), (d) and (e) only
3. (a), (b) and (c) only
4. (a), (c), (d) and (e) only
Subtopic: Â Cloning Vector |Â Tools |
 87%
Level 1: 80%+
NEET - 2022
Hints
Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
Subtopic: Â Tools |Â General Design of an rDNA experiment |
 75%
Level 2: 60%+
Please attempt this question first.
Hints
Please attempt this question first.
Which of the following DNA is used by Agrobacterium tumefaciens to transform normal cells into a tumor?
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
Subtopic: Â Transforming Plant & Animal Cell |
 89%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Selection of recombinations based on their inability to produce colour in the presence of a chromogenic substrate is
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
Subtopic: Â Cloning Vector |
 89%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Restriction endonucleases are bacterial enzymes that cut double stranded DNA. These restriction enzymes do not cut their own DNA because
| 1. | The bacterium does not contain the specific recognition sequence |
| 2. | The DNA is packed with histones |
| 3. | The specific recognition sequences are masked with methyl groups |
| 4. | Restriction endonucleases cut viral DNA only |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 84%
Level 1: 80%+
Please attempt this question first.
Hints
Consider the following steps involved in recombinant DNA technology
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
A. Downstream processing
B. Insertion of recombinant DNA into host cell
C. Isolation of genetic material
D. Obtaining the foreign gene product
E. Cutting of DNA at specific locations
Choose the correct sequence of steps
1. C, A, B, D, E
2. C, E, B, D, A
3. A, E, B, D, C
4. C, E, D, B, A
Subtopic: Â General Design of an rDNA experiment |
 80%
Level 1: 80%+
Hints
Given below are two statements, one is labelled as Assertion (A) and the other is labelled as Reason (R).
In light of the above statements, choose the correct answer from the options given below:
| Assertion (A): | When a particular restriction enzyme cuts strands of DNA, overhanging stretches or sticky ends are formed. |
| Reason (R): | Some restriction enzymes cut the strand of DNA a little away from the centre of palindromic site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True and (R) does not correctly explain (A). |
| 3. | (A) is True, (R) is False. |
| 4. | (A) is False, (R) is True. |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 61%
Level 2: 60%+
NEET - 2022
Hints
Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic: Â Separation and Isolation of DNA fragments |
 91%
Level 1: 80%+
NEET - 2022
Hints
Match List-I with List-II :
Choose the correct answer from the options given below:
| List I | List II | ||
| (a) | Gene gun | (i) | Replacement of a faulty gene by a normal healthy gene |
| (b) | Gene therapy | (ii) | Used for transfer of gene |
| (c) | Gene cloning | (iii) | Total DNA in the cells of an organism |
| (d) | Genome | (iv) | To obtain identical copies of a particular DNA molecule |
Choose the correct answer from the options given below:
| (a) | (b) | (c) | (d) | |
| 1. | (ii) | (i) | (iv) | (iii) |
| 2. | (i) | (iii) | (ii) | (iv) |
| 3. | (iv) | (i) | (iii) | (ii) |
| 4. | (ii) | (iii) | (iv) | (i) |
Subtopic: Â Transforming Plant & Animal Cell |
 92%
Level 1: 80%+
NEET - 2022
Hints
The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
1. (a) Cellulase (b) Protease
2. (a) Cellulase (b) Chitinase
3. (a) Chitinase (b) Lipase
4. (a) Cellulase (b) Lipase
Subtopic: Â General Design of an rDNA experiment |
 93%
Level 1: 80%+
NEET - 2022
Hints
© 2025 GoodEd Technologies Pvt. Ltd.